Published: 2022-08-05

Utilization of gelatin methacryloyl scaffolds in the regeneration of tooth and its supporting tissues

Kirthanashri S. Vasanthan, Deborah Sybil, Priyanshu Kumar Shrivastava, Tanveer Ahmad


Gelatin methacrylate (GelMA) due to its good biocompatibility, modifiable physical properties, and promotion of cellular adhesion, proliferation, and differentiation, serves as excellent scaffolds in tissue engineering and regeneration. These properties of GelMA make it a suitable choice for engineering of tooth and its supporting tissues. The complex spatial arrangement of the tooth along with the anisotropic nature of different tissues associated with it, make its regeneration challenging. Bioprinted GelMA scaffolds offer a great potential in mimicking the tooth architecture. In this study, GelMA scaffold was constructed by one pot method. The Fourier Transfer Infra red spectroscopy of GelMA confirmed the presence of amide bond at 1650 cm-1 (C=O stretching), 1550-1 (C-N stretching) and 1450-1. As evident from previous studies, the application of GelMA in whole tooth regeneration has not been studied exhaustively. However, GelMA has produced effective results in the regeneration of individual tooth components as well as the tooth germ. Therefore, this scaffold is proposed to be used in the whole tooth regeneration process because of its cytocompatiblity, cellular adhesion and tuneable mechanical properties.


Gelatin methacryloyl scaffolds, GelMA, Tooth regeneration, Tissue engineering

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Obregon F, Vaquette C, Ivanovski S, Hutmacher DW, Bertassoni LE. Three-dimensional bioprinting for regenerative dentistry and craniofacial tissue engineering. J Dent Res. 2015;94:143S-52S.

Ma Y, Xie L, Yang B, Tian W. Three‐dimensional printing biotechnology for the regeneration of the tooth and tooth‐supporting tissues. Biotechnology Bioengineering. 2019;116(2):452-68.

Zhang L, Morsi Y, Wang Y, Li Y, Ramakrishna S. Review scaffold design and stem cells for tooth regeneration. Japanese Dental Science Review. 2013;49(1):14-26.

Smith EE, Zhang WB, Schiele NR, Khademhosseini A, Kuo CK, Yelick PC. Developing a biomimetic tooth bud model. J Tissue Eng Regen Med. 2017;11:3326-36.

Monteiro N, Smith EE, Angstadt S, Zhang WB, Khademhosseini A, Yelick PC. Dental cell sheet biomimetic tooth bud model. Biomaterials. 2016;106:167-79.

Liu Y, Chan-Park MB. A biomimetic hydrogel based on methacrylated dextran-graft-lysine and gelatin for 3D smooth muscle cell culture. Biomaterials. 2010;31:1158-70.

Van den Steen PE, Dubois B, Nelissen I, Rudd PM, Dwek RA, Opdenakker G. Biochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9). Critical Reviews in Biochemistry and Molecular Biology. 2002;37:375-536.

Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A. Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials. 2010; 31:5536-44.

Khayat A, Monteiro N, Smith EE, Pagni S, Zhang W, Khademhosseini A, et al. GelMA-encapsulated hDPSCs and HUVECs for dental pulp regeneration. J Dental Res. 2017;96(2):192-9.

Hu Y, Wu Y, Gou ZY, Tao J, Zhang JM, Liu QQ, et al. 3D-engineering of cellularized conduits for peripheral nerve regeneration. Sci Rep. 2016;6:32184.

Chen X, Bai S, Li B, Liu H, Wu G, Liu S, et al. Fabrication of gelatin methacrylate/ nanohydroxyapatite microgel arrays for periodontal tissue regeneration. Int J Nanomedicine. 2016;11:4707.

Stratesteffen H, Kopf M, Kreimendahl F, Blaeser A, Jockenhoevel S, Fischer H. GelMA-collagen blends enable drop-on-demand 3D printablility and promote angiogenesis. Biofabrication. 2017;9.

Kurian AG, Singh RK, Patel KD, Lee JH, Kim HW. Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics. Bioact Mater. 2022;8:267-95.

Hölzl K, Lin S, Tytgat L, van Vlierberghe S, Gu L, Ovsianikov A. Bioink properties before, during and after 3D bioprinting. Biofabrication. 2016;8:032002.