DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20200196

Antibacterial efficacy of black seed honey in combination with penicillin and amoxiclav against gram-positive bacteria

Rozely Hossain, M. S. Rahman, M. A. Rayhan, Kashfia Nawrin, Mohammad M. Billah, M. R. Habib

Abstract


Background: The emergence of antimicrobial resistance possesses a great threat for the existence of mankind. Antibiotics like penicillin and amoxiclav are at the brink of losing their efficacy entirely in exposure to resistant bacteria. Thus, the present study was aimed to find out the antibacterial efficacy of black seed honey as an alternative natural source which can act independently and boost the efficacy of standard drugs alongside.

Methods: Penicillin, amoxiclav and black seed honey were first individually trailed against four gram-positive bacteria - Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Micrococcus luteus. Afterwards, penicillin and amoxiclav were used in combination with honey and compared the synergistic effects with their individual efficacy. Zones of inhibition from well diffusion method, percentage inhibition, minimum inhibitory and bactericidal concentrations by microdilution method were determined in the present study.  

Results: Black seed honey alone demonstrated great inhibitory potential against S. aureus (9.7 mm), S. epidermidis (9.9 mm) and M. luteus (9.3 mm) in well diffusion method. Moreover, its combination with amoxiclav showed synergistic effect against all bacteria except S. epidermidis. However, its conjugation with penicillin was not able to produce any synergism as exhibited by zones of inhibition. The lowest concentration (1.56%) of honey applied individually or in combination in microdilution method found highly effective which established an inverse dose dependent relationship with efficacy.

Conclusions: From the data it can be concluded that the black seed honey is a highly potent natural agent which can be utilized in antimicrobial therapy. However, further investigation is recommended to identify the responsible compound for such activity.


Keywords


Black seed honey, Well diffusion, Percentage inhibition, Minimum inhibitory, Minimum bactericidal concentration

Full Text:

PDF

References


Forouzanfar F, Bazzaz BS, Hosseinzadeh H. Black cumin (Nigella sativa) and its constituent (thymoquinone): a review on antimicrobial effects. Iran J Basic Med Sci. 2014;17(12):929-38.

Hegazi AG, Al Guthami FM, Al Gethami AFM, Allah FMA, Saleh AA, Fouad EA. Potential antibacterial activity of some Saudi Arabia honey. Vet World. 2017;10(2):233-237.

Olaitan PB, Adeleke OE, Ola IO. Honey: a reservoir for microorganisms and an inhibitory agent for microbes. Afr Health Sci. 2007;7(3):159-65.

Aurongzeb, M, Azim, MK. Antimicrobial properties of natural honey: a review of literature. Pak J Biochem Mol Biol. 2011;44(3):118-24.

Javadi SMR, Hashemi M, Mohammadi Y, MamMohammadi A, Sharifi A, Makarchian HR. Synergistic effect of honey and Nigella sativa on wound healing in rats. Acta Cir Bras. 2018;33(6):518-23.

Rayhan A, Yousuf SA, Rayhan J, Khengari EM, Nawrin K, Billah MM. Black seed honey—A powerful ingredient of prophetic medicine; its neuropharmacological potential. J Apither. 2019;5(2):18-26.

Sharma R1, Sharma CL, Kapoor B. Antibacterial resistance: Current problems and possible solutions. Indian J Med Sci. 2005;59(3):120-9.

Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci. 2018;25(2):361-6.

National Committee for Clinical Laboratory Standards (2017). Methods for determining bactericidal activity of antimicrobial agents. Tentative Guidelines, M26-TNCCLS, Villanova, PA. 2017.

Patton T, Barett J, Brennan J, Moran N. Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey. J Microbiol Methods. 2005;64:84-95.

Gambogou B, Khadimallah H, Bouacha M, Ameyapoh YA. Antibacterial activity of various honey monofloral and polyfloral from different region of Algeria against uropathogenic Gram Negative Bacilli. J Apither. 2018;4(1):1-8.

Bouacha M, Ayed H, Grara N. Honey bee as alternative medicine to treat eleven multidrug-resistant bacteria causing urinary tract infection during pregnancy. Sci Pharm. 2018;86(2):14.

Yocum RR, Rasmussen JR, Strominger JL. The mechanism of action of penicillin. Penicillin acylates the active site of Bacillus stearothermophilus D-alanine carboxypeptidase. J Biol Chem. 1980;255(9):3977-86.

National Center for Biotechnology Information. PubChem Database. Penicillin v, CID=6869. Available at https://pubchem.ncbi.nlm.nih.gov/ compound/Penicillin-v. Accessed on 28 October 2019.

Venugopalan V, Klinker, KP. Chapter 29: Cell Wall Inhibitors. In Lippincott® Illustrated Reviews: Pharmacology. 7th ed. Wolters Kluwer; 2019: 1047-1058.

Akhavan BJ, Vijhani P. Amoxicillin. In: Stat Pearls. Treasure Island (FL): Stat Pearls Publishing. Available at: https://www.ncbi.nlm.nih.gov/books/ NBK482250/. Accessed on 28 October 2019.

Finlay J, Miller L, Poupard JA. A review of the antimicrobial activity of clavulanate. J Antimicrob Chemother. 2003;52(1):18-23.

Stapleton P, Wu PJ, King A, Shannon K, French G, Phillips. Incidence and Mechanisms of Resistance to the Combination of Amoxicillin and Clavulanic Acid in Escherichia coli. Antimicrob Agents Chemother. 1995;39(11):2478-83.

Hamdy AA, Ismail HM, Al-Ahwal AM, Gomaa NF. Determination of flavonoid and phenolic acid contents of clover, cotton and citrus floral honeys. J. Egypt. Public Health Assoc. 2009;84:245-59.

Burns K. Health benefits of prophetic condiments: part one of five. Islam Online Arch 2000. Available at: https://archive.islamonline.net/?p= 15586. Accessed on 24 May 2019.

Alvarez-Suarez JM, Giampieri F, Battino M. Honey as a source of dietary antioxidants: structures, bioavailability and evidence of protective effects against human chronic diseases. Curr Med Chem. 2013;20:621-38.

Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005;26:343-56.