DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20203110

Detection of oral spirochete (Treponema denticola) by modified Fontana staining technique from sub-gingival plaques of patients suffering from periodontal disease and to analyse the morphology of spirochetes with respect to the disease’s status

Sunil Paudel, Jyoti M. Nagamoti, Ved Prakash Mishra, Suraj Twanabasu, Susan Kusma

Abstract


Background: Infections pertaining to mankind particularly those affecting the periodontal tissues are of serious concerns worldwide and are increasing on a global scale particularly in the tropical and subtropical countries like India. The clinical presentation, though very typical of periodontal infection, is often confused with other oral infection, making laboratory diagnosis and confirmation necessary. The management of periodontal infections needs personal hygiene, awareness of infection, proper diagnosis and medication. The present study was undertaken to demonstrate the oral spirochete (Treponema denticola) from periodontal pathogens suffering from periodontal disease.

Methods: A total of 55 clinically diagnosed selected patients of periodontal infection attending the outpatient Department of Periodontics, V. K. Institute of Dental Science, KLE University, Belgaum were studied. Dental plaque was taken as specimens from the patients were processed by modified Fontana staining and observed in microscope.  

Results: A total number of 55 plaque samples were stained by modified Fontana staining technique. 30 cases were positive and 25 cases were negative. Males were affected more as compared to the females and the age group ranging from 15 to 65. Farmers were more affected compared to other occupation.

Conclusions: Microscopic method by modified silver nitrate staining can be a very useful screening method for evaluation of oral spirochetes in a clinical setting if used judiciously keeping in mind the variables that can affect the results.


Keywords


Spirochetes, Modified Fontana stain, Treponema denticola, Periodontal disease

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References


Grossi SG, Genco RJ. Periodontal disease and diabetes mellitus: A two ways relationship. Ann Periodontol. 1998;3:51-61.

Loesche WJ. The role of spirochetes in Periodontal disease. Adv Dent Res. 1988;2(2):275-83.

Novak MJ. Classification of diseases and conditions affecting the periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA, editors. Carranza's Clinical Periodontology. 10th ed. St. Louis Missouri: Elsevier; 2007:103-104.

Rylev M, Kilian M. Prevalence and distribution of principal periodontal pathogens worldwide. J Clin Periodontol. 2008;35:346-61.

Saini S, Aparna, Gupta N, Mahajan A, Arora DR. Microbial flora in oro-dental infections. Indian J Med Microbiol. 2003;21:111-4.

Williams RC. Periodontal disease. New England J Med 1990;322(6):373-82.

Keyes PH, Likins RC. Plaque formation, periodontal disease, and dental caries in Syrian hamsters. J Dental Res. 1946;25:166.

Choi BK, Paster BJ, Dewhirst FE, Gobel UB. The Diversity Cultivable and Uncultivable Oral spirochetes from patients with severe destructive periodontitis. Infection Immunity. 1994;62(5):1889-95.

Listgarten MA, Levine S. Positive correlation between the proportions of subgingival spirochetes and motile bacteria and susceptibility of human subjects to periodontal deterioration. J Clin Periodontol. 1981;8:122-38.

Benachinmardi KK, Nagamoti J, Kothiwale S, Metgud SC. Microbial Flora in Chronic Periodontitis: Study at a Tertiary Health Care Centre from North Karnataka. J Lab Physicians. 2015;7(1):49-54.

Gangadhar NL, Rajashekar M. A modified Silver Impregnation staining for leptospires. Indian Vet J. 1998;75:349-51.

Bansode P, Deshpande S, Ahire C, Dekate K. Dental Caries Occurrence, Types and Occurrence of Trauma to Anterior Teeth, Their Severity in Relation to Pulpal Involvement in Patients Visiting O. P. D. of Government Dental College and Hospital, Aurangabad. J Evolution Med Dent Sci. 2014;3(44):10924-9.

Marrs JA, Trumbley S, Malik G. Early childhood caries: determining the risk factors and assessing the prevention strategies for nursing intervention. Pediatr Nurs. 2011;37(1):9-15.

Deepa B, Kishore B, Nayak R, Ingalgi P. Detection, semi-quantification and morphology of oral spirochetes in chronic periodontitis: a light microscopic study. J Pharm Biomed Sci. 2013;29(29):735-40.

Varsha Choudhary from Maratha Mandal’s NGH Institute of Dental Sciences and Research Centre Belgaum Karnataka India. Utility of silver nitrate staining in detection of spirochete from oral cavity of periodontally healthy and diseased individuals.

Cavrini F, Pirani C, Foschi F, Montebugnoli L, Sambri V, Pratic C. Detection of Treponema denticola in root canal systems in primary and secondary endodontic infections. A correlation with clinical symptoms. New Microbiol. 2008;31(1):67-73.

Scherp H. Current concepts in periodontal disease research: epidemiological contributions. J Am Dent Assoc. 1964;68:667-75.

Williams RC, Howell TH. Periodontology: section A: definition, epidemiology and etiology of periodontal disease. In: Shaw JH, Sweeney EA, Cappuccino CC, Meiler SM, eds. Textbook of oral biology. Philadelphia: W.B. Saunders; 1978: 1019-1031.

Loe H, Anerud A, Boysen H, Smith M. The natural history of periodontal disease in man: the rate of periodontal destruction before 40 years of age. J Periodontol. 1978;49:607-20.

Becker W, Berg L, Becker BE. Untreated periodontal disease: a longitudinal study. J Periodontol. 1979; 50:234-44.

Lindhe J, Haffajee AD, Socransky SS. Progression of periodontal disease in adult subjects in the absence of periodontal therapy. J Clin Periodontol. 1983;10:433-42.

Buckley LA, Crowley MJ. A longitudinal study of untreated periodontal disease. J Clin Periodontol. 1984;11:523-30.

Hausmann E, Dunford R, Wikesjo U, Christersson L, Henry MK. Progression of untreated periodontitis as assessed by subtraction radiography. J Periodontal Res. 1986;21:716-21.

Loe H, Anerud A, Boysen H, Morrison E. Natural history of periodontal disease in man: rapid, moderate and no loss of attachment in Sri Lankan laborers 14 to 46 years of age. J Clin Periodontol. 1986;13:431-45.

Epidemiology and Oral Disease Prevention Program, National Institute of Dental Research. Oral health of United States adults: The National Survey of Oral Health in U.S. employed adults and seniors, 1985-86: national findings. Bethesda, Md.: Department of Health and Human Services, Public Health Service, National Institutes of Health; 1987.

Socransky SS, Haffajee AD, Goodson JM, Lindhe J. New concepts of destructive periodontal disease. J Clin Periodontol. 1984;11:21-32.

Goodson JM, Acheal T, Haffajee AD, Somberger GC, Socransky SS. Patterns of progression and regression of advanced destructive periodontal disease. J Clin Periodontol. 1982;9:472-81.

Goodson JM, Haffajee AD, Socransky SS. The relationship between attachment level loss and alveolar bone loss. J Clin Periodontol. 1984;11:348-59.