DOI: http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20203112

Dynamics of change of chlorophyll and carotenoid pigments in leaves of Chrozophora tinctoria species spread in Nakhchivan Autonomous Republic (Azerbaijan Republic)

Samira F. Khudaverdiyeva

Abstract


Background: The pigment composition and the dynamics of pigment accumulation were investigated to determine the degree of adaptation of Chrozophora tinctoria to the environmental conditions in the Nakhchivan Autonomous Republic (AR) of Azerbaijan Republic.

Methods: The leaves were collected (transects size 5×5 m, in triplicate) and dried with 15-20 pieces, pigments were separated by solvent. The pigment density was determined by the absorption peak on a spectrophotometer, and then the pigment density was calculated.  

Results: The chlorophyll pigments in the dry weight of leaves varied between 2-6 mg and carotenoids 0.8-1.2 mg/g were found. Also, chlorophyll (a-b) pigments increased in 1.2 times, and carotenoids decreased in 3.6 times depending on environmental conditions.

Conclusions: Photosynthetic apparatus of the species actives in all cenosis with response adaptive to changing of environmental conditions depends from the time of leaf collection for the Chrozophora tinctoria species as shown results of researches.


Keywords


Chrosophora tinctora, Chlorophyll and carotenoid pigments, Accumulation level of pigments

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References


Maslova TG, Popova IA. Adaptive properties of the plant pigment systems. Photosynthetica. 1993;29:195-203.

Physiology of photosynthesis ed. Nichiporovich AA: Nauka M; 1982:320.

Young AJ, Britton G. Carotenoid sand stress. In: Alscher RG, Cumming JR (Eds.), Stress responses in plants: adaptation and acclimation mechanism. Wiley-Liss, New York, USA; 1990:87-112.

Mutygullina Y. Dynamics of the maintenance and role of pigments of photosynthesis at kinds of sort Dianthus L. florae Ciscaucasia Bulletin of Moscow State Regional University. Series: Natural Sci. 2009;1:52-5.

Peng Y, Gitelson AA, Sakarnolo I. Remote estimation of gross primary productivity in crops using MODIS 250 m data. Remote Sensing Environment. 2013;128:186-96.

Carnauchov VN. Biological functions of carotenoids. M: Nauka; 1988:240.

Porra RJ, Thompson WA, Kriedemann PE. Determination of accurate extinction coefficient and a simultaneous-equations for assaying chlorophyll-a and chlorophyll-b extracted with 4 different solvents verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta. 1989;975(3):384-94.

Welburn A. The spectral determination of chlorophyll-a and chlorophyll-b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiology. 1994;144(3):307-13.

Godnev TN. Structure of chlorophyll and methods for its quantitative determination. Minsk Pub NA BSSR; 1952:215.

Shlyk AA. About spectrophotometric determination of chlorophylls, a and b. Biochemistry. 1968;33(2):275-85.

Shlyk AA. Determination of chlorophylls and carotenoids in green leaves extracts//Biochemical metods in plant physiology. M: Nauka; 1971:154-170.

Titova MS, Rozlomiy NG. Dynamics of photosynthetic activity of the needles of Picea ajanensis and Picea smithiana in the green zone of c. Ussuriysk// “Living and biocoses systems”, Is.2; URL: http://www.jbks.ru/archive/issue-12/article-4; 2015. Accessed 10 May 2020.

Demmig B, Adams B, Gilmore AM, Adams WW. In vivo function of carotenoids in higher plants. FASEB J. 1996;10:403-12.

Novruzov EN, Aslanov SM, Mamedov CS, Shamsizade LA. TLC study of carotenoids Abstracts V Transcaucasian Conference. Adsorption Chromatography Baku. 1982:114-5.

Zhang C, Liu Y, Kovacs JM, Verdugo F, Santiago F, Chen K. Spectral response to varying levels of leaf pigments collected from a degraded mangrove forest. J Applied Remote Sensing. 2012;6:1-14.

Pogson BJ, Rissler HM, Frank HA. The roles of carotenoids in photosystem II of higher plants Photosystem II: the light driven water: plastoquinone oxidoreductase Eds. Wydrzynski T, Satoh K. Dordrecht: Springer Verlag; 2005:515-537.

Ritz T, Damnjanovic A, Schullen K, Zhang JP, Koyarna Y. Efficient light harvesting through carotenoids. Photosynthesis Res. 2000;66:125-44.